DNA Sequencing with TEM

Nanopore DNA sequencing with MspA.

Nanopore sequencing has the potential to become a direct, fast, and inexpensive DNA sequencing technology. The simplest form of nanopore DNA sequencing utilizes the hypothesis that individual nucleotides of single-stranded DNA passing through a nanopore will uniquely modulate an ionic current flowing through the pore, allowing the record of the current to yield the DNA sequence. We demonstrate ...

DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.

The highly thermostable DNA polymerase from Thermus aquaticus (Taq) is ideal for both manual and automated DNA sequencing because it is fast, highly processive, has little or no 3'-exonuclease activity, and is active over a broad range of temperatures. Sequencing protocols are presented that produce readable extension products greater than 1000 bases having uniform band intensities. A combinati...

DNA Sequencing

Double stranded DNA sequencing as a choice for DNA sequencing.

Double stranded DNA sequencing (1) is favored because of its simplicity, and convenice. However it is only recently that the quality of this method has become comparable with single stranded DNA sequencing. We believe that two factors have limited the popularity of double stranded DNA sequencing: 1, the quality of the template DNA; 2, the inherent propertv.,of the DNA polymerase. The modified T...

DNA sequencing: clinical applications of new DNA sequencing technologies.

We are in a time of great change in genetics that may dramatically impact human biology and medicine. The completion of the human genome project,1,2 the development of low-cost, high-throughput parallel sequencing technology, and large-scale studies of genetic variation3 have provided a rich set of techniques and data for the study of genetic disease risk, treatment response, population diversi...